Quantitative Reverse Transcription-PCR Comparison of Tumor Cell Enrichment Methods
نویسندگان
چکیده
منابع مشابه
Quantitative reverse transcription-PCR comparison of tumor cell enrichment methods.
platelet plots are not always seen in cryoglobulinemia, although other interferences are usually detected with high efficiency. For comparison, whereas the Technicon H*3 appeared to provide a more immediate visual indication of sample cryoglobulins, large platelet count overestimates were still obtained in most cases. Examination of graphic plots is not used in all laboratories, and its efficie...
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Over the past few years high-throughput platforms for real-time quantitative PCR have become widely available. The cost of RNA extraction from a large number of samples are, however, quite notable. One method that stands out with respect to free up- or downscaling of sample size and reliability is the isolation of mRNA using oligodeoxythymidylate [oligo(dT)25]-coated magnetic particles. In comb...
متن کاملDesign and optimization of reverse-transcription quantitative PCR experiments.
BACKGROUND Quantitative PCR (qPCR) is a valuable technique for accurately and reliably profiling and quantifying gene expression. Typically, samples obtained from the organism of study have to be processed via several preparative steps before qPCR. METHOD We estimated the errors of sample withdrawal and extraction, reverse transcription (RT), and qPCR that are introduced into measurements of ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2001
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/47.10.1860